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HOME > J Yeungnam Med Sci > Volume 38(3); 2021 > Article
Review article
Lactate: a multifunctional signaling molecule
Tae-Yoon Leeorcid
Yeungnam University Journal of Medicine 2021;38(3):183-193.
DOI: https://doi.org/10.12701/yujm.2020.00892
Published online: February 18, 2021

Department of Microbiology, Yeungnam University College of Medicine, Daegu, Korea

Corresponding author: Tae-Yoon Lee, MD, PhD Department of Microbiology, Yeungnam University College of Medicine, 170 Hyeonchung-ro, Nam-gu, Daegu 42415, Korea Tel: +82-53-640-6941 Fax: +82-53-653-6628 E-mail: doxr7p@gmail.com
• Received: December 22, 2020   • Revised: January 15, 2021   • Accepted: January 25, 2021

Copyright © 2021 Yeungnam University College of Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Since its discovery in 1780, lactate has long been misunderstood as a waste by-product of anaerobic glycolysis with multiple deleterious effects. Owing to the lactate shuttle concept introduced in the early 1980s, a paradigm shift began to occur. Increasing evidence indicates that lactate is a coordinator of whole-body metabolism. Lactate is not only a readily accessible fuel that is shuttled throughout the body but also a metabolic buffer that bridges glycolysis and oxidative phosphorylation between cells and intracellular compartments. Lactate also acts as a multifunctional signaling molecule through receptors expressed in various cells and tissues, resulting in diverse biological consequences including decreased lipolysis, immune regulation, anti-inflammation, wound healing, and enhanced exercise performance in association with the gut microbiome. Furthermore, lactate contributes to epigenetic gene regulation by lactylating lysine residues of histones, accounting for its key role in immune modulation and maintenance of homeostasis.
Single-celled organisms, such as bacteria and fungi, are put under pressure to reproduce as soon as they encounter nutrient-rich environments. Their metabolism control systems have evolved to sense available nutrients and transfer the necessary carbon, nitrogen, and energy to generate building blocks for cell growth. When starved, the cells cease to produce biomass and begin adapting their metabolism to draw maximum energy from available resources to cope with nutrient depletion [1].
In multicellular organisms, uncontrolled proliferation is prevented as mammalian cells cease to take up nutrients from the environment unless otherwise stimulated by growth factors. Genetically mutated cancer cells, on the other hand, are non-dependent on such external stimuli and may show increased uptake and metabolism of nutrients, promoting cell survival and proliferation [1]. Oncogenic mutations often result in a high-rate uptake of nutrients, particularly glucose, which is required for cell growth and proliferation [2]. In 1923, Otto Warburg found that when glucose was added to cancer tissue slices in Ringer’s solution, they continuously produced lactate in the presence of oxygen [3,4]. His attempt was to understand why cancer cells preferred glucose fermentation over oxidative phosphorylation (OXPHOS), even in the presence of ample oxygen. This aerobic glycolysis occurs not only in cancer cells but also in exercising muscle cells, activated immune cells, and virally infected cells. A glycolytic increase in lactate concentration is found in local tissues under various disease conditions, such as sepsis, infections, inflammatory diseases, autoimmune diseases, and cancer.
Lactate has long been regarded as a by-product of anaerobic respiration. Increasing evidence, however, indicates that lactate is essential for energy and redox homeostasis. More recent data suggest that lactate is a multifunctional signaling molecule with its own receptor and plays an important role in communication between cells and tissues. The aim of this review was to provide knowledge on lactate, including its general physiology and historical paradigm changes, and to summarize the recent advances that highlight the regulatory role of lactate in diverse biological processes in both physiological and pathological conditions.
Almost 99% of lactic acid is dissociated into lactate anions (La) and protons (H+) within the physiological pH range. Therefore, in this review, La will be referred to as lactate. Lactate is mainly composed of L (+) enantiomer (L-form) as detected in the sera of healthy humans [5]. Serum D-lactate concentration ranges from 0.013 to 0.2 mM, as opposed to that of L-lactate which may range from slightly above 1.14 mM at rest, 15 mM after an intense exercise, and 30 mM in some cancer tissues [6]. The daily production of lactate in resting humans is estimated to be approximately 20 mmol/kg/day [7]. In adults with an average body weight of 70 kg, approximately 1,400 mmol of lactate is produced daily from muscle (25%), skin (25%), brain (20%), red blood cells (20%), and intestines (10%) [8].
Lactate is produced by the reduction of pyruvate by lactate dehydrogenase (LDH) during glycolysis under anaerobic or aerobic conditions.
CH3COCOOPyruvate+NADH+H+ LDH CH3CHOHCOOLactate+NAD+
LDH is a tetrameric enzyme that catalyzes the interconversion of pyruvate and lactate with the complementary interconversion of a reduced form of nicotinamide adenine dinucleotide (NADH) to NAD+. The two most common subunits of LDH are the LDHM (or M subunit) and LDHH (or H subunit) proteins that are encoded by LDHA and LDHB genes, respectively. These subunits combine to form five isoenzymes: LDH-1 (4H), LDH-2 (3H1M), LDH-3 (2H2M), LDH-4 (1H3M), and LDH-5 (4M) [9]. LDH-1 is usually called LDHB since it is composed of four “H” LDHB subunits. LDHB preferentially converts lactate to pyruvate and is expressed mainly in the heart (H). In contrast, LDH-5 is usually called LDHA since it is composed of four “M” LDHA subunits. LDHA preferentially converts pyruvate to lactate and is expressed mainly in the skeletal muscle (M) [10]. Heterogenous LDHs (LHH-2 to LDH-4) exhibit subunit-dependent intermediate enzyme activity. The remaining two subunits, LDH-C and LDH-Bx, are specific to the testes and peroxisomes, respectively. In lactate-producing glycolysis, LDHA is the most important enzyme.
Lactate has long been considered a waste end product of anaerobic glycolysis [11-14]. The development of acidosis has traditionally been explained by lactate overproduction during high-intensity exercises or severe disease states such as sepsis and septic shock [15]. A high level of serum lactate has been regarded as a predictive factor of muscle fatigue, often associated with tissue hypoxia and poor clinical outcomes [16].
The early 1980s was marked by a drastic change in the lactate paradigm. The idea that lactate is not responsible for acidosis started gaining acceptance, as reviewed by Robergs et al. [17]. According to the theory, intermediate acids of glycolysis have a low pKa; hence they all exist in their base form. The first intermediate acid of glycolysis, 3-phosphoglyceric acid, is present as 3-phosphoglycerate. This signifies that the main form of glycolytic metabolites is not acid (pyruvic acid or lactic acid) but a base (pyruvate or lactate). For example, at physiological pH (~6.4 to 7.1), lactic acid, due to its very low acid dissociation constant (pKa, ~3.86), dissociates immediately into lactate and hydrogen ion (H+). This indicates that lactic acid does not exist in living organisms. In fact, lactate does not decrease metabolic acidosis, but rather removes H+ from the cytosol and creates NAD+ molecules, electron acceptors that are used for adenosine triphosphate (ATP) generation to maintain glycolysis [18,19].
How, then, is lactate removed? To maintain the serum concentration range of 1 to 2 mM, 60 to 120 mmol of lactate needs to be removed from the blood every hour [20]. Lactate is usually removed immediately from various tissues, for example, skeletal muscles, or it may be released and taken up by exercising muscle, heart, brain, kidney, and liver. Lactate may also be metabolized either by direct oxidation or transformation into glucose. Lactate transformation into glucose by the liver or kidney is known as the Cori cycle or gluconeogenesis [21]. Lactate can also be removed via its oxidation into pyruvate, which involves an entry into the tricarboxylic acid (TCA) cycle needed for ATP production [22,23].
As the product of glycolysis and the substrate for OXPHOS or gluconeogenesis, lactate can be regarded as the link between different metabolic pathways. In 1985, George Brooks [24] proposed the lactate shuttle hypothesis. Lactate shuttles and metabolic interplay are now recognized to mediate redox and energy homeostasis not only between cells, tissues, and at the whole organism level but also at an intracellular level between cell compartments, such as cytosol-mitochondria or cytosol-peroxisome shuttle. The early lactate shuttle reported in various cells, tissues, and organs, including muscle, heart, and liver [24-27], has now extended to those of sperm [28], adipose tissue [29], the brain [30], and the lungs [31-33]. Approximately half of the available lactate is removed through oxidation at rest, and up to 80% during exercise [34].
In contracting skeletal muscle, for example, lactate production and utilization occur simultaneously. It has been proposed that myocytes have two metabolic compartments, one for glycolysis (cytoplasm) and the other for oxidative metabolism (mitochondria) [35]. According to this concept, cytosolic lactate enters mitochondria via lactate transporters (intracellular lactate shuttle) and is oxidized to pyruvate through the mitochondrial lactate oxidation complex at the mitochondrial inner membrane, composed of mitochondrial LDH, CD147, and cytochrome oxidase [35]. In the myocardium, lactate uptake increases during conditions such as exercise, β-adrenergic stimulation, fast-pacing, and shock. Lactate may account for up to 60% of the cardiac oxidation substrate [36]. In the human brain, lactate accounts for approximately 7% of the energy requirement during rest, which increases up to 25% during exercise [37]. The lactate from the blood is either oxidized by neurons or converted and stored as glycogen. The lactate from glycolysis of the stored glycogen in astrocytes is transported into neurons (referred to as astrocyte-neuronal lactate shuttle) and oxidized to pyruvate and utilized by the TCA cycle [38,39].
Lactate is transported across cytoplasmic and intracellular compartments by several monocarboxylate transporters (MCTs) that perform proton-lactate symport. Among the 14 MCTs that have been identified to date, MCT1–4 are better characterized and have been shown to mediate the proton-linked transport of monocarboxylates such as lactate, pyruvate, acetoacetate, and β-hydroxybutyrate. MCT1 (also known as SLC16A1) is widely distributed and is usually involved in the import of lactate, whereas MCT4 (also known as SLC16A3) is expressed in highly glycolytic cells or tissues, such as white skeletal muscle, astrocytes, cancer cells, and white blood cells, and is mainly involved in lactate export [39,40]. In addition to MCTs, sodium-coupled lactate transport is carried out by the high-affinity transporter SLC5A8 or the low-affinity transporter SLC5A12 as was initially reported in the kidney [41]. The expression of SLC5A12 has also been reported in CD4+ T cells [42].
Lactate is a ligand for an orphan G-protein coupled receptor 81 (GPR81), now termed hydroxycarboxylic acid receptor 1 (HCAR-1 or HCA1) [39]. HCA1 is predominantly expressed in the adipose tissue. Other tissues and organs also express HCA1, although to a lesser degree, in the skeletal muscle, liver, spleen, kidney, and brain. In addition to the plasma membrane, HCA1 is also detected in intracellular organelles, such as mitochondria. As expected from the broad presence of the lactate receptor HCA1 in various tissues, increasing evidence has shown that lactate plays diverse roles in various pathophysiological conditions, including inflammation and cancer. HCA1-mediated lactate signaling may affect lipid metabolism [43], neuronal excitability changes [44], cellular development and survival [45,46], and modulation of inflammatory responses [47,48]. Lactate generated by glycolytic cancer cells can also act on HCA1 expressed on non-cancer cells, including immune cells, endothelial cells, adipocytes, and fibroblasts in the tumor stroma. The final consequence of lactate-mediated activation of HCA1 on cells in the tumor mass is the facilitation of survival, growth, and metastasis of cancer cells via mechanisms that mediate increased angiogenesis, immune evasion, and chemoresistance [49].
Lactate signaling via HCA1 has been shown to work synergistically with insulin to decrease cellular concentration of cyclic adenosine monophosphate (cAMP) and lipolysis in the fed state, suggesting that HCA1 might be linked to obesity [29]. The mechanism of cAMP modulation includes attenuation of protein kinase A (PKA) signaling [50,51]. Lactate produced and released by inflammatory bone marrow neutrophils may induce mobilization via endothelial HCA1 signaling by inhibiting the expression of VE-cadherin in intercellular junctions of the vascular endothelium [52].
Lactate binding to HCA1 can also signal through a noncanonical, cAMP/PKA-independent pathway with arrestin beta 2 (ARRB2) as an adaptor protein, leading to the inhibition of toll-like receptor-4 (TLR-4)- and nucleotide-binding oligomerization domain (NOD)-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome-mediated induction of proinflammatory mediators such as interleukin (IL)-1β and IL-18 [47,48,53].
Normal cells obtain ATP, an essential energy source for cell survival, from both glycolysis and mitochondrial OXPHOS. In 1923, Otto Warburg and Seigo Minami [3] found high rates of glycolysis in cultured tumor tissues, which were characterized by increased glucose uptake and excessive lactate production even in the presence of oxygen (aerobic glycolysis). They incubated slices of rat hepatoma in Ringer’s solution and found an increase in lactate concentration calculated from the increase in CO2 during 30 minutes of incubation. The rate of lactate production from the hepatoma slice was 70 times greater than that in the normal liver, kidney, and heart tissue [54]. Warburg [4] also showed, in a rat model, that the artery feeding the tumor had lower levels of lactate and higher levels of glucose than the draining vein from the tumor tissue. This glucose-avid, lactate-producing behavior of tumor cells regardless of oxygen availability was named the “Warburg effect” by Efraim Racker in 1972 [55].
Mitochondrial function is not impaired in Warburg cells, as described in many types of cancers; the TCA cycle is activated in breast cancer, glioblastoma, and non-small cell lung cancer cells [56]. Therefore, the Warburg effect is a metabolic shift from (not a defect of) OXPHOS to glycolysis. Glycolysis generates only two molecules of ATP per glucose molecule, which is much less efficient than OXPHOS (36 ATPs per glucose). Nevertheless, most cancer cells obtain approximately 60% of the total required ATP from glycolysis. The activation of glycolysis has several advantages for rapidly proliferating cancer cells. In addition to the faster ATP synthesis than OXPHOS, the activation of the glycolytic pathway allows cells to feed several pathways that contribute to macromolecular synthesis. These include the pentose phosphate pathway, which produces ribose for nucleotides and NADPH for reductive biosynthesis, the hexosamine pathway, which is required for protein glycosylation, serine-glycine-one-carbon metabolism, which feeds glutathione, nucleotides, and methylation reactions; and glycerol synthesis for the production of complex lipids [57].
One of the key components of glycolytic activation and OXPHOS suppression is hypoxia-inducible factor 1α (HIF-1α) [58], which was originally identified as a critical transcription factor for cellular adaptation to hypoxic conditions. HIF-1α induces most of the glycolytic enzymes, including hexokinase 2, pyruvate kinase M2 (PKM2), and LDHA. HIF-1α also activates the expression of glucose transporter (GLUT) 1 and GLUT3 and a lactate transporter MCT4. HIF-1α activates pyruvate dehydrogenase (PDH) kinase 1, which phosphorylates and inactivates PDH, the enzyme that converts pyruvate to acetyl coenzyme A (acetyl-CoA), which is essential for the TCA cycle.
Warburg and Minami [3] observed almost a century ago that Warburg-type cancer is characterized by the excessive production and accumulation of lactate. Tumors are a heterogeneous collection of normal cells, cancer cells, immune cells, and blood vessels. Even among cancer cells, some are glycolytic and others are oxidative in the context of vascularization and preferred metabolism. Extracellular lactate excreted from glycolytic stromal cells in the tumor tissue may be taken up by adjacent oxidative cancer cells, via MCTs, and be converted to pyruvate to enter the TCA cycle, which produces electron donors, such as NADH and reduced flavin adenine dinucleotide to support the electron transport chain (ETC) for ATP production. This type of two-compartment communication of tumor metabolism was termed the “reverse Warburg effect” as aerobic glycolysis takes place in tumor stromal fibroblasts rather than cancer cells [59]. The mitochondrial reactive oxygen species (ROS) produced by the ETC activity of reverse Warburg cells are released into the cytosol and inhibit prolyl hydroxylase (PHD) through the Fenton reaction and prevent the degradation of HIF-1α [60]. The stabilization of HIF-1α activates the transcription of glycolytic genes, thus converting the oxidative cells to glycolytic ones. Lactate can, in turn, promote HIF-1α stabilization by inhibiting PHD activity. Lactate-derived pyruvate competitively inhibits alpha-ketoglutarate, a PHD co-factor, from associating with PHD [61]. Recent findings indicate that lactate preconditioning also primes normal fibroblasts to switch from OXPHOS to glycolysis through mechanism(s) including ROS-mediated HIF-1α stabilization [62]. PKM2 is a rate-limiting enzyme that catalyzes the conversion of phosphoenolpyruvate to pyruvate. PKM2 enzyme activity is allosterically regulated by the oligomerization type. The PKM2 tetramer is enzymatically more active than the dimer form of PKM2. However, the PKM2 dimer acts as a transcriptional coactivator, which may translocate to the nucleus and promote the expression of HIF-1α-mediated pro-glycolytic genes such as lactate-producing LDHA [63]. Thus, lactate, the final product of normoxic glycolysis, further activates glycolysis through the activation of HIF-1α. In addition to hypoxia, the increase in several metabolic intermediates, such as succinate, fumarate, and lactate, facilitates the stabilization of HIF-1α. The oxygen-independent mechanism of HIF-1α activation is now termed pseudohypoxia [64].
Hypoxia and glycolysis have long been considered to activate the immune cells. For example, a metabolic shift from OXPHOS to aerobic glycolysis is regarded as a hallmark of T cell activation [65]. LDHA induced in activated T cells to support glycolysis promotes interferon (IFN)-γ expression and instead maintains high levels of acetyl-CoA to increase histone acetylation and transcription of ifng [66].
Recent studies have highlighted the counterbalancing regulatory function of the glycolysis metabolite lactate. In addition to its metabolic functions as an energy source and an intermediate metabolite for biosynthetic pathways, lactate also plays a modulatory role in inflammation and immunity. Recent reports suggest that lactate produced by aerobic glycolysis has an immunosuppressive effect in the local environment of various disease conditions including sepsis, cancer, chronic inflammation, and autoimmune diseases [67-69]. In sepsis, immunosuppression is a serious problem that causes life-threatening secondary infections and is now called immunoparalysis [70]. The immunosuppressive phase during sepsis is characterized by the depletion of effector cells and T cell exhaustion, and a concomitant increase in regulatory T cells (Tregs) and myeloid-derived suppressor cells [71]. Lactate has also been shown to suppress the proliferation and cytokine production in human cytotoxic T cells [72], thereby decreasing the cytotoxic effect. Effector T cells are more dependent on glycolysis for proliferation and cytokine production, while Tregs rely more on OXPHOS [73]. Lactate accumulation in the local tissue environment is a common feature of both inflammatory diseases and cancer. Lactate in the tumor microenvironment (TME) has been shown to help tumor escape from immune surveillance by reshaping T cells and macrophages to immunosuppressive phenotypes such as tumor-promoting Tregs and M2-like tumor-associated macrophages (TAMs) [72-76].
In contrast, increased lactate concentration observed in the local tissue of chronic inflammatory diseases contributes to the upregulation of the sodium-coupled lactate transporter SLC5A12 by human CD4+ T cells. In a mouse arthritis model, lactate was shown to promote IL-17 production by CD4+ T cells through phosphorylation of signal transducer and activator of transcription 3 (STAT3) by PKM2 and fatty acid synthesis (FAS). It also led to CD4+ T cell retention in the inflamed tissue by reducing the glycolysis rate and diverting metabolic fluxes into de novo FAS [68]. Lactate has been shown to inhibit antigen presentation and IL-12 synthesis by dendritic cells (DCs). The differentiation of DCs from monocytes is also affected by high concentrations of lactate, favoring less inflammatory DCs that are biased toward producing IL-10 [76,77].
Macrophages may undergo a switch in the metabolic pathways, which leads to differentiation into either a proinflammatory (M1) or a homeostatic and anti-inflammatory (M2) phenotype in response to various stimuli, including cytokines [78]. In general, IFNs promote M1-like inflammatory macrophage activation by suppressing homeostatic pathways. In the context of tumors, TAMs do not completely follow M1 and M2 subtypes. TAMs usually have an M2-like phenotype and facilitate tumor growth by immunosuppression. Signaling functions and polarization of M2 macrophages can be directly regulated by lactate in the TME via HIF-1α-dependent metabolic reprogramming [78]. The lactate produced by cancer cells further facilitates HIF-1α stabilization and induces an M2-like phenotype, such as vascular endothelial growth factor (VEGF). It has been shown that lactate promotes M2 polarization of macrophages by binding to the lactate receptor HCA1 [48].
Viral RNA can be detected by retinoic acid-inducible gene 1 (RIG-I)-like receptors (RLRs), such as RIG-I and melanoma differentiation-associated gene 5 (MDA5), which subsequently activate the mitochondrial antiviral signaling (MAVS) protein and downstream axis for type I IFN production [79]. Cells infected by RNA viruses often show an increase in the rate of glycolysis and a decrease in OXPHOS activity, which is a favorable state for rapid viral replication [80]. Furthermore, lactate induced by glycolytic activation inhibits RLR-mediated IFN production [81,82]. Upon recognizing cytosolic double-stranded RNAs, RIG-I undergoes conformational changes, oligomerization, and exposure of the two N-terminal caspase activation and recruitment domains (CARDs) that are involved in a CARD-CARD interaction with MAVS. The transmembrane domain (TM) at the C-terminus of MAVS is required for its localization at the mitochondrial outer membrane. Upon activation, MAVS develops a functional prion-like structure, which serves as a platform for the MAVS signalosome for the activation of the type I IFN pathway. Intriguingly, a recent report showed that lactate inhibits this antiviral axis by directly binding to the TM domain of MAVS, disrupting the mitochondrial localization of MAVS, RIG-I-MAVS interaction and downstream signaling, and IFN-β activation [82] (Fig. 1).
Surprisingly, lactate can act as a precursor for the epigenetic lactylation of histone lysine residues, which stimulates gene transcription from chromatin. Histones are proteins that are critical in the packing of DNA into the cell and into chromatin and chromosomes. Histones may experience posttranslational modifications in their protruding N-terminal tails as well as within the C-terminal region. Various histone modifications, including acetylation, methylation, and ubiquitylation, were reported before the discovery of histone lactylation in 2019 [83]. These changes in the amino acids of histone proteins can affect gene expression as well as overall chromatin condensation. A total of 28 lysine lactylation sites have been identified on the core histones in human and mouse cells. For example, in macrophages stimulated to produce lactate by hypoxia, IFNγ plus lipopolysaccharide, or bacterial challenge, histone lactylation accumulated at their promoters is associated with the shift from the initial M1-like phenotype to induction of M2-like homeostatic genes at late time points (16–24 hours) after stimulation. Histone lactylation does not occur at the promoters of proinflammatory genes, such as Tnf or Il6. The induced M2-like genes include Arg1, which is involved in wound healing. Thus, it has been suggested that an endogenous “lactate clock” in M1 macrophages challenged by bacteria turns on the homeostatic genes at the end of the inflammatory phase. This is a new feedback mechanism that restrains macrophage activation [84]. Epigenetic regulation of metabolism and immunity by histone lactylation extends our understanding of the roles of lactate under diverse conditions.
In healing wounds, cells divide rapidly to activate the glycolytic pathway, resulting in lactate accumulation in the interstitial fluids up to a range of 5–15 mM [39,85]. Lactate has been shown to promote reparative angiogenesis through mechanisms including recruitment of endothelial progenitor cells, stimulation of endothelial cell migration, activation of procollagen factors, and enhancement of collagen deposition in the extracellular matrix. Lactate induces the release of mediators such as VEGF, IL-1, and transforming growth factor beta (TGF-β), all of which consequently stimulate angiogenesis and promote wound healing [86-88]. Exogenous lactate delivery is expected to be helpful in the management of non-healing wounds [88].
The human gut microbiota is linked to various health and disease states. Many studies have indicated that the gut microbiota profile is related to the prevalence of chronic diseases such as diabetes mellitus and metabolic syndrome [89]. Iraporda et al. [90] showed that lactate downregulates proinflammatory responses in intestinal epithelial and myeloid cells. They suggested beneficial effects of lactate-containing foods, such as kefir, on gut microbiota in general and also showed their protective role against infection by pathogenic bacteria such as Salmonella sp. [91,92]. Lactate is an end product of bacterial fermentation in many lactic acid bacteria (i.e., Lactobacillus and Bifidobacterium), which are regarded as key members of the healthy gut microbiota. Lactate produced by Lactobacillus and Bifidobacterium in the colon is rapidly converted into short-chain fatty acids, such as propionate, butyrate, and succinate. Lactate produced in the gut may also be released into the systemic circulation via MCTs and removed or metabolized in distant organs [93]. On the other hand, lactate produced by exercising skeletal muscle may be released into circulation and can be transported into the gut lumen where lactate-utilizing microbiota strains are present. This type of novel communication of lactate between the gut and somatic organs was proposed as gut-soma lactate shuttle [32,33].
Many studies on the athlete’s gut microbiota have shown distinct microbial compositions, including elevated abundances of Veillonella, Bacteroides, Prevotella, Methanobrevibacter, and Akkermansia [94,95]. A bacterial strain belonging to Veillonella atypica was isolated by Scheiman et al. [96] from stool samples of elite marathon runners postmarathon who ran in the 2015 Boston Marathon. Intriguingly, inoculation of this strain into mice significantly increased the treadmill run time by 13%. They found that V. atypica, which utilizes lactate as its sole carbon source, metabolizes lactate into propionate and acetate, as detected by metagenomic analysis. The 13C3-labeled lactate tracing experiment in mice showed that serum lactate crosses the epithelial barrier into the lumen of the gut [96]. This study revealed that lactate, a metabolic by-product of aerobic exercise, maybe shuttled into the gut lumen via circulation and provide a carbon source to V. atypica, a gut microbiota that can then convert it to a bioactive propionate that improves exercise performance.
Lactate treatment can be helpful in several clinical conditions. For example, lactated Ringer’s (LR) solution is one of the most popular crystalloid fluids for patients with trauma, burns, and surgery. LR contains sodium lactate, which is known to be a metabolic fuel that improves cardiac function. In vivo, lactate is rapidly metabolized into bicarbonate by the liver [97]. In a recent study using a neonatal hypoxia-ischemia model, lactate administration reduced the extent of the brain lesion and facilitated behavioral recovery [98]. The usefulness of lactate administration has also been reported in wound healing [88] and muscle regeneration after injury [99].
Increased lactate levels may also be related to maladaptive pathological conditions. For example, lactate accumulation in the TME is associated with cancer progression and poor clinical outcomes, such as resistance to chemotherapy, increased metastasis, and immune evasion [74,75]. Lactate is often monitored as a prognostic indicator of severe disease conditions, such as sepsis. It should be noted that increased lactate production is due not only to hypoxia or tissue hypoperfusion but also multiple factors. In addition to hypoxia, serum lactate increases due to increased production during stress conditions (e.g., intense exercise, asthma, and sepsis) and decreased clearance of lactate by the liver and kidney. The lactate levels of patients with asthma or hypertension should be interpreted based on their medication information. Since β2-adrenergic stimulators may induce upregulation of lactate production, serum lactate levels can be blunted in patients using β2-adrenergic receptor blockers [8].
Since Warburg’s historical finding of aerobic glycolysis in cancer cells, lactate has long been regarded as a culprit of muscle fatigue and acidosis-induced tissue damage. However, it is now accepted that lactate is a useful metabolic fuel for skeletal muscles, heart, and brain. Lactate also functions as a metabolic buffer that links glycolysis and OXPHOS. The introduction of the lactate shuttle concept in the early 1980s vastly changed the lactate paradigm and revealed that lactate is a ubiquitous molecule that is metabolized and used almost everywhere in the body. Lactate shuttles were identified between astrocytes and neurons, contracting and resting skeletal muscles, glycolytic and oxidative cancer cells, and intracellular compartments such as the cytosol and mitochondria. The discovery of the lactate receptor GPR81/HCA1 further extended the landscape of lactate’s playing grounds, spanning adipose tissue, skeletal muscles, liver, kidney, heart, brain, immune cells, endothelium, and stromal fibroblasts in the tumor mass. The receptor-mediated nonmetabolic effects of lactate include immunosuppression, active participation in wound healing with increased angiogenesis, decreased adipocyte lipolysis, and neuroprotection through anti-inflammatory activities. Lactate overproduced during intense exercise has been shown to cross the epithelial barrier into the gut lumen, increasing the relative abundance of a strain of V. atypica. Metabolic conversion of lactate into propionate by V. atypica has been shown to increase exercise performance. Finally, lactate is involved in the epigenetic regulation of gene expression by lactylating histones, which contributes to immune modulation and maintenance of homeostasis.
Lactate, regarded for a long time as a metabolic waste product since its discovery, is now recognized as a fuel energy source, a precursor of gluconeogenesis, a signaling molecule, a regulator of gene expression, a precursor for exercise-enhancing propionate production by gut microbiota, and probably as more unknowns yet to be explored.

Conflicts of interest

No potential conflict of interest relevant to this article was reported.

Fig. 1.
The metabolism, shuttle, transporters, receptor, and diverse functions (green boxes) of lactate in glycolytic cells. See text for details. GPR81, G-protein coupled receptor 81; HCA1, hydroxycarboxylic acid receptor 1; ECM, extracellular matrix; ARRB2, arrestin beta 2; Gi, inhibitory G protein; ATP, adenosine triphosphate; cAMP, cyclic adenosine monophosphate; PKA, protein kinase A; MCT, monocarboxylate transporter; GLUT1, glucose transporter 1; NAD, nicotinamide adenine dinucleotide; NADH, reduced form of NAD; LDHA, A form of lactate dehydrogenase (LDH); ETC, electron transport chain; acetyl-CoA, acetyl coenzyme A; FADH2, reduced flavin adenine dinucleotide; TCA, tricarboxylic acid cycle; MAVS, mitochondrial antiviral signaling; RIG-I, retinoic acid-inducible gene 1; dsRNA, double-stranded RNA; IFN, interferon.
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    Figure

    JYMS : Journal of Yeungnam Medical Science