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J Yeungnam Med Sci > Volume 20(2); 2003 > Article
Yeungnam University Journal of Medicine 2003;20(2):142-151.
DOI:    Published online December 31, 2003.
Bone Formation by rhBMP-7 Transduced HEK 293 Cells in Nude Mouse.
Su Yon Jeong, Won Tae Chang, Yon Sil Chang, Myun Hwan Ahn, Jae Ryong Kim, In Hwan Song
1Department of Anatomy, College of Medicine, Yeungnam University, Daegu, Korea.
2Department of Biochemistry.Molecular biology, College of Medicine, Yeungnam University, Daegu, Korea.
3Department of Orthopedic surgery, College of Medicine, Yeungnam University, Daegu, Korea.
To induce bone formation at ectopic site by tissue engineering and gene therapy, we transplanted collagen sponges containing rhBMP-7 transduced HEK 293 cells in the hypodermis of nude mice. Bone formation was investigated by histological and electron microscopic method at 3, 6, and 9 weeks after transplantation. At 9 weeks after transplantation, eosinophilic bony tissue was observed in the implanted collagen sponge and was confirmed as bone tissue by Von Kossa stain. In the transmission electron microscopic observation, the cells in newly formed bone tissue had eccentrically located nucleus and well developed rough endoplasmic reticulum (rER). Therefore, the cells were evaluated as osteoblasts. Those results suggest that it is possible to form a bone tissue in the ectopic site by transplantation of rhBMP-7 transduced HEK 293 cells. This will be contributed to push more advanced gene therapy for bone formation. However, the HEK 293 cell is unable to apply to the clinical gene therapy. Therefore it is worth to find more compatible cells for clinical application. In addition, collagen sponge is considered as an excellent scaffold and/or carrier for gene therapy and a good biomaterial for tissue engineering.
Key Words: BMP-7, Osteoinduction, Collagen sponge
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