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JYMS : Journal of Yeungnam Medical Science

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Joo Young Kim 2 Articles
In Vitro Culture of Endothelial Cell and Smooth Muscle Cell for Studying Vascular Diseases.
Joo Young Kim
Yeungnam Univ J Med. 2010;27(2):91-97.   Published online December 31, 2010
DOI: https://doi.org/10.12701/yujm.2010.27.2.91
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AbstractAbstract PDF
Endothelial cells play a key role in pathological processes such as cancer cell metastasis, atherosclerosis, and diabetic retinopathy. Vascular smooth muscle cells directly involve in the formation of atheroma in atherosclerosis. Some kinds of the endothelial cells are simply harvested from the umbilical veins, the tunica intima of aortic walls, the retina using various enzymes solutions. Those purely isolated cells provide a powerful tool in vitro studies of the endothelial cell related diseases. In this context, the cultured smooth muscle cells after the isolation from the tunica media of aortic walls are also used for elucidating the pathogenesis of atherosclerosis. Here, I briefly introduce articles that include the isolation of human umbilical vein endothelial cells (HUVEC), aortic endothelial and smooth muscle cells, retinal microvascular endothelial cells (RMEC), as well as the diseases' applications of these cells.
Isolation of Endothelial Cells and Smooth Muscle Cells from Rat Aort.
Young Eun Yun, In Hwan Song, Eon Ki Sung, Joo Young Kim
Yeungnam Univ J Med. 2006;23(2):182-192.   Published online December 31, 2006
DOI: https://doi.org/10.12701/yujm.2006.23.2.182
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AbstractAbstract PDF
BACKGROUND
Atherosclerosis has emerged as the leading cause of death in developed countries. At present, human umbilical vein endothelial cells (HUVEC) are most commonly used for the investigation of Endothelial cells (EC). However, HUVEC are not found in arteries but only in veins. Currently there are many reports on methods used to isolate EC;, most of these methods require special equipment to remove contaminating smooth muscle cells (SMC). MATERIALS AND METHODS: The method described here may be used to isolate not only ECs but also SMCs;,the approach presented here did not require special equipment. Rat aorta was treated with 2 mg/ml of type II collagenase solution for 45 minutes. The isolated cells from the aorta were incubated in medium G for a week;, only ECs could be separated. After the collagenase treatment, the rest of aorta was cut lengthwise, and left undisturbed to obtain SMCs in the culture dish for 10 days. To verify the purity of the isolated cells, we performed immunofluorescence and evaluated the results with transmission electron microscopy analysis. RESULTS: The immunofluorescence study demonstrated specific expression of CD31 and alpha-smooth muscle actin in the isolated ECs and SMCs, respectively. Cultured ECs and SMCs showed their own fine structure characteristics. CONCLUSION: These results suggest that this method for isolating ECs and SMCs may be especially useful for the study of atherosclerosis.

JYMS : Journal of Yeungnam Medical Science