- In Vitro Culture of Human Nasal Epithelial Cells by Monolayer Culture of Dissociated Cells.
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Yong Dae Kim, Si Youn Song, Myung Ki Min, Jang Su Suh, Kei Won Song, Ho Sun Park
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Yeungnam Univ J Med. 1998;15(2):286-296. Published online December 31, 1998
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DOI: https://doi.org/10.12701/yujm.1998.15.2.286
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- Different techniques for culturing respiratory epithelial cells have been developed to overcome the limitations of studies on in vivo and on bioptic material. Traditionally, culture systems are divided into organ cultures, explant cultures and dissociated cell cultures. The first two contain both epithelial and non-epithelial cells. However, in monolayer cultures of dissociated cells only epithelial cells are present, the effects observed are caused by a pure epithelial responses. The purpose of this study is to establish primary culture method of human nasal epithelium (HNEC) by monolayer culture of dissociated cells to evaluate the role of the epithelial cells in the allergic and non-allergic nasal inflammatory reactions. HNEC was prepared by primary culture method of monolayer culture of dissociated cells from human inferior nasal turbinate mucosa of septal deviation patients. Primary cultured cells were characterized by indirect immunofluorescence assay and transmission electron microscopy. The immunoreactivities of cytokeratin-pan and cytokeratin No. 8 were observed in cultured HNEC. However, the immnoreactivities of vimentin and von Willebrand factor were not observed in cultured HNEC. The tonofilaments and desmosome were observed in cultured HNEC. The cultured epithelial cells were identified to be pure nasal epithelial cells. The monolayer culture of dissociated cells could successfully be employed for further study to investigate the role of the epithelial cells in allergic or non-allergic nasal inflammatory diseases.
- Pharyngoesophageal reconstruction.
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Gyu Ho Cha, Jeong Cheol Kim, Kyung Ho Lee, Dong Bo Suh, Jang Su Suh
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Yeungnam Univ J Med. 1992;9(1):167-174. Published online June 30, 1992
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DOI: https://doi.org/10.12701/yujm.1992.9.1.167
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- Microvascular tissue transfers have facilitated primary closure of various complex defects after radical ablation of head and neck cancers. From Oct 1991 to Feb 1992, we used forearm free flap in two patients and delto-pectoral flap in one patient who had preoperative irradiation for pharyngoesophageal reconstruction. The stricture and fistula formation were most troublesome complication in forearm free flap, so we designed as lazy S shape in distal flap margin to prevent circular contraction and longitudinal margin was deepithelialized (5 mm) and sutured double layer to withstand fistular formation and this can be considered useful in place of a free jejuna transfer.
- Clincal Effect of Ketotifen in Allergic Rhinitis.
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Sun Jin Eun, Yong Dae Kim, Mun Heum Park, Jang Su Suh, Kei Won Song
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Yeungnam Univ J Med. 1990;7(1):105-112. Published online June 30, 1990
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DOI: https://doi.org/10.12701/yujm.1990.7.1.105
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- Ketotifen, a benzocycloheptathiophene, has an orally effective antiallergic as well as antihistaminic properties. In pervious studies, Ketotifen has shown encouraging results on patient with allergic rhinitis, either perennial or seasonal. 39 patients with allergic rhinitis had been treated with Ketotifen 1 mg twice daily for 8 weeks. And we obtained following results. 1) The efficacy rate in sneezing attack was 73.5%, in nasal discharge 71%, in nasal obstruction 58%. 2) Some improvements in at least one of three-major symptoms were noted within 1 week in 30.7%, within 2 weeks in 55.8%, within 3 weeks in 66.7%, within 8 weeks in 87.2%. 3) Physical findings such as colour, swelling of turbinate, character of rhinorrhea were not improved significantly. 4) Side effect was observed only in one patient with abdominal pain and diarrhea, which was subsided after interruption of administration. These results suggested that Ketotifen was effective in treatment of allergic rhinitis.
- A Case of the Inverted Papilloma with Verrucous Carcinoma of the Nose and Paranasal Sinuses.
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Myeong Soo Jang, Mun Heum Park, Jang Su Suh
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Yeungnam Univ J Med. 1989;6(2):279-285. Published online December 31, 1989
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DOI: https://doi.org/10.12701/yujm.1989.6.2.279
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- Inverted Papilloma arising from mucous membrane of the nasal cavity and paranasal sinuses is rare benign neoplasm, and usually unilateral arise from lateral wall of nasal cavity. This tumor is histologically benign neoplasm but clinically malignant, because of frequent recurrence and extensive bone destruction. And occasionally, this tumor can be transformed to squamous cell carcinoma or verrucous carcinoma. Recently, we have experienced a case of the inverted papilloma with verrucous carcinoma of the nose and paranasal sinuses and patient expired due to extension of intracranial cavity. We report out case with review of current literatures.
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