Journal of Yeungnam Medical Science

Original article

Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells: Sohee Kang; Yeungnam Univ J Med. 2020;37(3):217-225. Published online May 22, 2020; DOI: https://doi.org/10.12701/yujm.2020.00248

5,985 View
141 Download
8 Crossref

Background
To provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs).
Methods
Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials.
Results
BD showed the highest calcium release in all test periods, followed by PR and TC. (p<0.05). All experimental groups showed high alkalinity after 1 day, except at 14 days. BD showed the highest cell viability compared with PR and TC after 1 and 3 days, while TC showed the lowest value (p<0.05). The CLSM analysis showed that cells were well adhered and expressed actin filaments for all pulp capping materials. Mineralization by PR and BD groups was higher than that by TC group based on alizarin red S staining. BD showed significantly higher alkaline phosphatase activity than PR and TC, while TC showed the lowest value (p<0.05).
Conclusion
Within the limitations of the in vitro study, BD had higher mineralization-inducing potential than PR and TC.

Citations

Citations to this article as recorded by

Effect of pulp capping materials on odontogenic differentiation of human dental pulp stem cells: An in vitro study
Mahmoud M. Bakr, Mohamed Shamel, Shereen N. Raafat, Robert M. Love, Mahmoud M. Al‐Ankily
Clinical and Experimental Dental Research.2024;[Epub] CrossRef
Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts
Gabriel Kato, Rita Araújo, Cláudia Rodrigues, Pedro Sousa Gomes, Liliana Grenho, Maria Helena Fernandes
Journal of Functional Biomaterials.2023; 14(6): 314. CrossRef
Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study
Monthip Wannakajeepiboon, Chankhrit Sathorn, Chatvadee Kornsuthisopon, Busayarat Santiwong, Thanakorn Wasanapiarnpong, Pairoj Linsuwanont
BMC Oral Health.2023;[Epub] CrossRef
Cytotoxicity of dental self-curing resin for a temporary crown: an in vitro study
Jae-wan Ko, Joon Sakong, Sohee Kang
Journal of Yeungnam Medical Science.2023; 40(Suppl): S1. CrossRef
Ion release, biocompatibility, and bioactivity of resin-modified calcium hydroxide cavity liners
Nastaran Taghvaei, Mehrsima Ghavami-Lahiji, Mehdi Evazalipour, Reza Tayefeh Davalloo, Ehsan Zamani
BMC Oral Health.2023;[Epub] CrossRef
Morphogenesis of Osteoid Structures during Cultivation of Mesenchymal Stromal Cells on Fibrillary Collagen in the Presence of Silicoaluminophosphate
A. A. Gaidash, M. I. Blinova, S. A. Aleksandrova, Yu. A. Nashchekina, V. K. Krutko, O. N. Musskaya, K. V. Skrotskaya, A. V. Nashchekin, N. A. Mikhailova, A. I. Kulak
Cell and Tissue Biology.2022; 16(1): 52. CrossRef
Present status and future directions: Hydraulic materials for endodontic use
Josette Camilleri, Amre Atmeh, Xin Li, Nastaran Meschi
International Endodontic Journal.2022; 55(S3): 710. CrossRef
The scientific management of deep carious lesions in vital teeth using contemporary materials—A narrative review
M. Al-Ali, J. Camilleri
Frontiers in Dental Medicine.2022;[Epub] CrossRef

First
Prev
Page of 1
Next
Last

Search