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JYMS : Journal of Yeungnam Medical Science

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Sung Kweon Son 1 Article
Membrane protein alterations associated with anticancer drug resistance in mouse lymphoblastic leukemia L1210 cells
Seong Yong Kim, Sung Kweon Son, Jae Ryong Kim, Jung Hye Kim
Yeungnam Univ J Med. 1993;10(2):432-444.   Published online December 31, 1993
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AbstractAbstract PDF
Multidrug resistance (MDR) phenotype is frequently observed in animal and human cancer cell lines selected for in vitro resistance to a single chemotherapeutic agent. It is characterized by the diminished j drug accumulation and is related to the drug efflux mechanism in resistant cells. In the present study, adriamycin resistant cells (L1210-AdR6: 10-6M adriamycin, -AdR5: 10-5M) and vincristine resistant cells (L1210-VcR7: 10-7M vincristine, -VcR6: 10-6M) were produced from mouse lymphoblastic leukemia cell line L1210. Growth profiles of survived cells were observed for 5 days with MTT (thiazolyl blue) assay and resistance was compared with IC50 (drug concentration of 50% survival reduction in absorbance). Resistant cells proliferated more slowly than sensitive cell. Doubling times were 29.7hr in L1210, 68.7 hr in L1210-AdR5 and 58.2 hr in -VcR6. MDRs expressed as resistance factor were as follows, L1210-AdR5 was 76.4 times for vincristine, L1210-VcR6 was 96.4 times for adriamycin. The cell membrane proteins with three different M.W. were recognized to be related resistance, 220, 158, and 88 Kd in L1210-AdR5, 158, 140 and 88 Kd in L1210-VcR6 by SDS-PAG electrophoresis. Cell surface membrane proteins were identified by radio-iodination and autoradiogram. Their molecular weights were 158, 72.8, and 42.4 Kd in L1210-VcR6.


Citations to this article as recorded by  
  • Suppression of P-glycoprotein expression by antipsychotics trifluoperazine in adriamycin-resistant L1210 mouse leukemia cells
    Soon Young Shin, Byeong Hyeok Choi, Jae-Ryong Kim, Jung-Hye Kim, Young Han Lee
    European Journal of Pharmaceutical Sciences.2006; 28(4): 300.     CrossRef

JYMS : Journal of Yeungnam Medical Science