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Jae Ho Jeong 8 Articles
The Effects of Autologous Serum on the Attachment and Proliferation of Human Primary Chondrocyte Culture
Jae Ho Jeong
Yeungnam Univ J Med. 2007;24(2 Suppl):S555-561.   Published online December 31, 2007
DOI: https://doi.org/10.12701/yujm.2007.24.2S.S555
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AbstractAbstract
Background
:Culture method for expanding chondrocytes ex vivo is an important procedure in cartilage tissue engineering. In most laboratories related to tissue engineering, fetal bovine serum is widely used as supplement. However, the chondrocytes grown in a medium containing fetal bovine serum may cause infectious viral diseases such as Creutzfeldt-Jacob disease and/or unfavorable immune reaction to bovine proteins. As a way out of these problems, we examined whether a patient's autologous serum could support the growth and attachment of his/her chondrocytes. Materials and Methods:Chondrocytes were isolated from microtia patients (age between 5 to 12) by enzymatic digestion and cultured in a medium supplemented with 10% autologous serum, 10% fetal bovine serum (FBS) and 10% banked allogenic serum respectively. Proliferation and attachment rate were assessed by Trypan blue staining and MTT assay. Attachment rate was checked at 6, 12, 24, 36, 48 hours after plating of cells and counting was done with hemocytometer after trypan blue exclusion. Proliferation rate was checked at 1, 3, 5, 7, 9 days after plating of cells and measurement was done with cell counting and MTT assay.
Results
:As a result, chondrocytes which were cultured in the medium supplemented with 10% autologous serum, represented higher rate of both proliferation and attachment, which is comparable to the chondrocytes in the culture supplemented with 10% FBS. But, chondrocytes in the culture supplemented with 10% banked allogenic serum showed lower rate of proliferation and attachment.
Conclusion
:The beneficial effect of autologous serum which has been confirmed in this study is another important progress for clinical application of tissue engineering. The possibility of banked allogenic serum is still remained. In this study, we used banked allogenic serum which containing anti-coagulants and this component may have affected on the result. Fresh allogenic serum should be utilized for next step of experiment.
Progress of Techniques in Breast Reconstruction Using Autologous Abdominal Tissue.
Jae Ho Jeong
Yeungnam Univ J Med. 2006;23(1):1-9.   Published online June 30, 2006
DOI: https://doi.org/10.12701/yujm.2006.23.1.1
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AbstractAbstract PDF
Breast reconstruction provides dramatic improvement for patients with severe deformity. The reconstruction not only restores aesthetically acceptable breast for patients with mastectomy deformity but also recovers psychological trauma of 'losing feminity' after the cancer mastectomy. There are many options for breast reconstruction from simple prosthetic insertion to a flap operation using autologous abdominal tissue. The choice of operation method depends on the physical condition of the patient, smoking habits, and economic status. Among the many options, the method that uses the lower abdominal tissue is known as the TRAM (transverse rectus abdonimis myocutaneous) flap. Since the introduction of the TRAM flap in 1982 by Hartrampf, the art of breast reconstruction using lower abdominal tissue has been progressively refined to pedicle flap, muscle-sparinga TRAM flap, and recently there have been exciting and revolutionary changes associated with the adoption of the concept of perforator flap. This refined method of breast reconstruction utilizes lower abdominal tissue nourished by the deep inferior epigastric perforator (DIEP). With the DIEP free flap, almost all of the rectus muscle and anterior rectus sheath are preserved and the donor morbidity is minimized. Different from previous flap methods using lower abdominal tissue, DIEP free flap method preserves function of the rectus muscle completely. Understanding the entire progression of breast reconstruction methods using lower abdominal tissue is necessary for plastic surgeons; the understanding of each step of the exciting progression and the review of the past history of the TRAM flap may provide insight for future development.
Reconstruction of Large Heel Defects Using Gracilis Muscle Free Flaps.
Jae Ho Jeong
Yeungnam Univ J Med. 1997;14(1):227-236.   Published online June 30, 1997
DOI: https://doi.org/10.12701/yujm.1997.14.1.227
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  • 1 Citations
AbstractAbstract PDF
No abstract available.

Citations

Citations to this article as recorded by  
  • Perspectives on reconstructive microsurgery in Korea
    Byung-Joon Jeon, Goo-Hyun Mun
    Journal of the Korean Medical Association.2011; 54(6): 604.     CrossRef
Resurfacing of the Open Wound of the Hand with Free Arterialized Venous Falp.
Sang Hyun Woo, Seong Eon Kim, Jae Ho Jeong, Kyung Ho Lee, Jung Hyun Seul
Yeungnam Univ J Med. 1994;11(2):303-313.   Published online December 31, 1994
DOI: https://doi.org/10.12701/yujm.1994.11.2.303
  • 1,314 View
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AbstractAbstract PDF
Since introduction of venous flap in 1980, many experimental studies and clinical applications of various kinds of venous flaps were reported. Venous flap has the following advantages : (1) nonbulky and goo-quality of flap (2) long & large vascular pedicle (3) easy & rapid elevation of flap (4) no sacrifice of major arteries (5) a single operative field. But, we also have some disadvantages of difficult handling of the pliable veins and the tmcertainty of flap survival. For the better result we had to design the size of the flap larger than that of defect and increase the number of draining vein to reduce the postoperative edema of the flap. We have treated the defects of soft tissue of the hand using free arterialized venous flap from the flexor aspect of the forearm & had an excellent results.
A Study for Reducing Pain from Injection of Lidocaine Hydrochloride.
Jae Ho Jeong, Kyung Ho Lee
Yeungnam Univ J Med. 1994;11(1):30-34.   Published online June 30, 1994
DOI: https://doi.org/10.12701/yujm.1994.11.1.30
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AbstractAbstract PDF
Local anesthetics produce pain during infiltration into skin. The relationship between local anesthetic-induced pain and pH of the local anesthetic solution has not been fully investigated. Commercial preparation of local anesthetics are prepared as acidic solutions of the salts to promote solubility and stability. And the acidity of local anesthetic solition may be related with the pain during infiltration of the solutione. So, we tried to neutralize the lidocaine hydrochloride solution which is one of the most frequently used local anesthetic agent. Sodium bicarbonate was used for neutralization. Sodium bicarbonate was mixed with lidocaine hydrochloride until the resulting pH of the solution become 7.4 which is identical to the acidity of body fluid. To identify the effect of neutralized lidocaine solution, we had a course of double blind test to 6 volunteers. Both forearm of each volunteer were injected with neutralized lidocaine and plain one and the degree of pain was estimated by each volunteers. According to subjective description by the volinteers, everyone felt neutralized lidocaine injection site was less painful than plain lidocaine. We concluded that we could reduce pain from infiltration of lidocaine hydrochloride by neutralization of the anesthetic solution with sodium bicarbonate.
Abdominoplasty.
Jung Hyun Seul, Jae Ho Jeong
Yeungnam Univ J Med. 1993;10(2):287-297.   Published online December 31, 1993
DOI: https://doi.org/10.12701/yujm.1993.10.2.287
  • 1,111 View
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AbstractAbstract PDF
No abstract available.
Augumentation Rhinoplasty Using Autogenous Cranial Bone Graft.
Jeong Cheol Kim, Sang Hyun Woo, Jae Ho Jeong, See Ho Choi, Jung Hyun Seul
Yeungnam Univ J Med. 1989;6(1):133-140.   Published online June 30, 1989
DOI: https://doi.org/10.12701/yujm.1989.6.1.133
  • 1,222 View
  • 6 Download
AbstractAbstract PDF
Augumentation rhinoplasty using autogenous cranial bone graft (outer table) can be used more successfully than other methods. In patients with congenital or posttraumatic severe saddle nose deformity and lateral deviation, cranial bone graft is an excellent method of augumentation. The advantages of cranial bone graft compared with traditional method of bone graft are summarized as follows; 1. Easy to reach donor site 2. Abundance of material 3. Little pain and functional disability 4. Shorter hospitalization period 5. Inconspicuous donor scar 6. No secondary deformity of donor site 7.Appropriate curvature can be obtained by proper selection of donor site. With the above advantages, we conclude that augumentation rhinoplasty using split cranial bone graft is a good method in correction of congenital or posttraumatic deformity of nose.
Histologic Changes of Rabbit Skin Induced by Progressive Tissue Expansion.
Jae Ho Jeong, Ki Yeol Kim, See Ho Choi, Jung Hyun Seul
Yeungnam Univ J Med. 1988;5(2):25-30.   Published online December 31, 1988
DOI: https://doi.org/10.12701/yujm.1988.5.2.25
  • 1,209 View
  • 3 Download
AbstractAbstract PDF
Soft-tissue expansion is a new surgical technique of providing donor tissue in modern reconstructive surgery. This technique provides a quantity of tissue of similar color, texture, and hair-bearing qualities for reconstruction of adjacent defects. It is known that the expanded skin shows several constant histologic changes including the increase in collagen fibers and vascularity within dermis, and thinning of subcutaneous tissue and dermis. In this study, the author observed serial histologic changes of rabbit skin induced by progressive tissue expansion up to excessive expansion of 6 times. The results are as follows: 1. Changes in the thickness of the epidermis was minimal until 3 times of expansion, but slight thinning was observed at excessive expansion state. 2. The thickness of the dermis was progressively decreased, and collagen fibers in the dermis was rapidly increased in early phase of expansion. 3. The vascularity in the dermis was also progressively increased. 4. The skin appendages showed no structural changes even in excessive expansion. 5. The panniculus carnosus showed no atrophic changes and the thickness was maintained in excessive expansion.

JYMS : Journal of Yeungnam Medical Science